Maybach Music Group Presents Self Made, Vol. 2 Various Artists 2012
About the Author: Buzzbin Mike
I'm the guy behind Buzzbin Magazine. Back in 2008 I put out my first issue of Buzzbin hoping to promote the local music scene in Akron/Canton. Since then we've grown to be the largest alternative press in the area. If you've met any of our crew then you know that we a bunch of hard workers with a passion to represent the community. Who says Akron/Canton/Cleveland is a miserable place to live? Not us. We have one of the best art scenes around, some of the hardest working musicians on the planet, and really great people to fill in everywhere else. We put out a new issue on THE FIRST WEDNESDAY OF EVERY MONTH covering the best art and entertainment found in northeast Ohio. We never miss a beat. Wonder why our mag is choked full of advertisers? Because we know what we're doing. We offer full scale marketing for each and every business that comes to us. We develop marketing campaigns from the ground up, or we'll take over where someone left off. We have the ability to tell everyone about you. And we're open for business. We're not pushy, we're helpful. - Mike (330) 236-5005
Antisense approach promising for treatment of parasitic infectionsPublic release date: 13-Aug-2012 [ | E-mail | Share ]
Contact: John Easton john.easton@uchospitals.edu 773-795-5225 University of Chicago Medical Center
A targeted approach to treating toxoplasmosis, a parasitic disease, shows early promise in test-tube and animal studies, where it prevented the parasites from making selected proteins. When tested in newly infected mice, it reduced the number of viable parasites by more than 90 percent, researchers from the University of Chicago Medicine report in the Proceedings of the National Academy of Sciences.
This precisely focused therapy combines short strands of "antisense" nucleic acid-like material with a small peptide that can transport those strands through cell membranes and into parasites, where they disrupt genetic signals. A similar approach from a team at Yale, published in April, showed comparable promise as a treatment for the parasites that cause malaria.
"This was proof of concept," said study author Rima McLeod, MD, a toxoplasmosis expert and professor at the University of Chicago Medicine. "We were able to cross multiple membranes, to insert the antisense strands into parasites living within cells and prevent them from making several different proteins. We now think we can shut down any of this parasite's genes."
"This approach may even have a role in non-parasitic diseases," she added. It is currently being tested in drug-eluting stents, as a treatment for bacterial or viral infections, including Ebola, and in patients with Duchenne muscular dystrophy, where it can block production of the defective segment of a dysfunctional gene.
The parasite McLeod and colleagues focused on, Toxoplasma gondii, is "probably the most common parasitic infection in the world," she said. "It infects as many as one-third of all humans, about two billion people worldwide." T. gondii causes disease in those who have immature immune systems, particularly those infected in utero. It also can be devastating for those who are immune-compromised and when it causes eye disease.
"New medications are urgently needed," she said. The standard treatments can cause side effects and patients may become hypersensitive to them. There are no medicines that can eliminate certain latent stages of the parasite's life cycle. There is no vaccine for humans.
The new treatment consists of a phosphorodiamidate morpholine oligomer (PMO), a short DNA-like molecule that binds to messenger RNA, preventing it from being translated into protein. This is conjugated to a "transductive peptide," a small molecule that can ferry the PMO across cellular barriers. The combination is known as a PPMO. An earlier study from the McLeod lab showed that such transductive peptides could bring small molecules into the untreatable dormant phase of the parasite.
The researchers tested this system in infected cells in tissue culture and in live, recently infected mice. It was able to knock down production of several distinct proteins.
They first tested their PPMO against easily detectable biomarkers by inserting genes for yellow fluorescent protein and for luciferase, a protein responsible for fireflies' glow, into parasites. Then they exposed parasite-infested cells to low levels of a PPMO targeting one piece of those genes. This reduced yellow fluorescence or dimmed bioluminescence by 40 to 60 percent.
Next, they tested its ability to block production of an enzyme, dihydrofolate reductase (DHFR), that the parasite needs to make folate and to replicate. After 48 hours, DHFR production of intracellular parasites was markedly reduced. Antisense oligomers targeting another enzyme and factors that direct the activity of many genes, called "transcription factors," associated with parasite replication, "also were successful," the authors note, "reducing parasite replication."
When they tested the anti-DHFR PPMO in newly infected mice, the results were dramatic. Within 96 hours, treatment reduced the number of parasites by 83 percent to 97 percent, depending on the measurement technique.
This approach is "paradigm shifting," McLeod said. "It has the potential to abrogate any molecular target and underscores the variety of diseases for which such an approach might apply."
The technology still has a few problems, she said. These PPMOs have a narrow therapeutic index; they can be toxic at a little more than the lowest effective dose. And we have not yet developed a way to eradicate latent stages of T. gondii, which can lie dormant in retina or brain cells for years, but such studies are underway. The researchers hope to use this technique to awaken dormant parasites, and then use drugs or PPMOs that target replicating parasites to kill them or eliminate both active and dormant parasite stages by targeting their plant-like transcription factors and other proteins unique to the parasites that humans don't have.
Much of the credit for developing and testing this system should go to the study's lead author, McLeod said.
Bo-Shiun Lai, a 20-year-old rising fourth-year college student at the University of Chicago, stumbled into his research position in McLeod's laboratory but adapted quickly. "Although it was difficult at the outset," he said, "everyone in the lab helped me develop the project and hone my laboratory techniques along the way."
It paid off. He completed his senior thesis as a junior, with honors, and will be applying for PhD programs.
###
This study was funded by the National Institutes of Health and gifts from the Mann and Cornwell, Taub, Rooney-Alden, Engel, Pritzker, Harris, Zucker, Morel and Mussilami families. Additional authors include Kamal El Bissati, Ying Zhou, Ernest Mui and Alina Fomovska of the University of Chicago, and William Witola, now at Tuskeegee University in Alabama.
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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Antisense approach promising for treatment of parasitic infectionsPublic release date: 13-Aug-2012 [ | E-mail | Share ]
Contact: John Easton john.easton@uchospitals.edu 773-795-5225 University of Chicago Medical Center
A targeted approach to treating toxoplasmosis, a parasitic disease, shows early promise in test-tube and animal studies, where it prevented the parasites from making selected proteins. When tested in newly infected mice, it reduced the number of viable parasites by more than 90 percent, researchers from the University of Chicago Medicine report in the Proceedings of the National Academy of Sciences.
This precisely focused therapy combines short strands of "antisense" nucleic acid-like material with a small peptide that can transport those strands through cell membranes and into parasites, where they disrupt genetic signals. A similar approach from a team at Yale, published in April, showed comparable promise as a treatment for the parasites that cause malaria.
"This was proof of concept," said study author Rima McLeod, MD, a toxoplasmosis expert and professor at the University of Chicago Medicine. "We were able to cross multiple membranes, to insert the antisense strands into parasites living within cells and prevent them from making several different proteins. We now think we can shut down any of this parasite's genes."
"This approach may even have a role in non-parasitic diseases," she added. It is currently being tested in drug-eluting stents, as a treatment for bacterial or viral infections, including Ebola, and in patients with Duchenne muscular dystrophy, where it can block production of the defective segment of a dysfunctional gene.
The parasite McLeod and colleagues focused on, Toxoplasma gondii, is "probably the most common parasitic infection in the world," she said. "It infects as many as one-third of all humans, about two billion people worldwide." T. gondii causes disease in those who have immature immune systems, particularly those infected in utero. It also can be devastating for those who are immune-compromised and when it causes eye disease.
"New medications are urgently needed," she said. The standard treatments can cause side effects and patients may become hypersensitive to them. There are no medicines that can eliminate certain latent stages of the parasite's life cycle. There is no vaccine for humans.
The new treatment consists of a phosphorodiamidate morpholine oligomer (PMO), a short DNA-like molecule that binds to messenger RNA, preventing it from being translated into protein. This is conjugated to a "transductive peptide," a small molecule that can ferry the PMO across cellular barriers. The combination is known as a PPMO. An earlier study from the McLeod lab showed that such transductive peptides could bring small molecules into the untreatable dormant phase of the parasite.
The researchers tested this system in infected cells in tissue culture and in live, recently infected mice. It was able to knock down production of several distinct proteins.
They first tested their PPMO against easily detectable biomarkers by inserting genes for yellow fluorescent protein and for luciferase, a protein responsible for fireflies' glow, into parasites. Then they exposed parasite-infested cells to low levels of a PPMO targeting one piece of those genes. This reduced yellow fluorescence or dimmed bioluminescence by 40 to 60 percent.
Next, they tested its ability to block production of an enzyme, dihydrofolate reductase (DHFR), that the parasite needs to make folate and to replicate. After 48 hours, DHFR production of intracellular parasites was markedly reduced. Antisense oligomers targeting another enzyme and factors that direct the activity of many genes, called "transcription factors," associated with parasite replication, "also were successful," the authors note, "reducing parasite replication."
When they tested the anti-DHFR PPMO in newly infected mice, the results were dramatic. Within 96 hours, treatment reduced the number of parasites by 83 percent to 97 percent, depending on the measurement technique.
This approach is "paradigm shifting," McLeod said. "It has the potential to abrogate any molecular target and underscores the variety of diseases for which such an approach might apply."
The technology still has a few problems, she said. These PPMOs have a narrow therapeutic index; they can be toxic at a little more than the lowest effective dose. And we have not yet developed a way to eradicate latent stages of T. gondii, which can lie dormant in retina or brain cells for years, but such studies are underway. The researchers hope to use this technique to awaken dormant parasites, and then use drugs or PPMOs that target replicating parasites to kill them or eliminate both active and dormant parasite stages by targeting their plant-like transcription factors and other proteins unique to the parasites that humans don't have.
Much of the credit for developing and testing this system should go to the study's lead author, McLeod said.
Bo-Shiun Lai, a 20-year-old rising fourth-year college student at the University of Chicago, stumbled into his research position in McLeod's laboratory but adapted quickly. "Although it was difficult at the outset," he said, "everyone in the lab helped me develop the project and hone my laboratory techniques along the way."
It paid off. He completed his senior thesis as a junior, with honors, and will be applying for PhD programs.
###
This study was funded by the National Institutes of Health and gifts from the Mann and Cornwell, Taub, Rooney-Alden, Engel, Pritzker, Harris, Zucker, Morel and Mussilami families. Additional authors include Kamal El Bissati, Ying Zhou, Ernest Mui and Alina Fomovska of the University of Chicago, and William Witola, now at Tuskeegee University in Alabama.
[ | E-mail | Share ]
?
AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Portable air conditioners are small sized and are extremely convenient as they can be easily moved from one room into the other. Portable models however have other advantages as well.
It is quite difficult to live in the absence of constant air conditioning, but this comes with a certain price. A portable air conditioner is a viable solution, as it is easy to move it between rooms or sites. Thus, it is a great cooling solution for homes or offices.
Portable air conditioners can be easily installed and can be moved from one place or room into the other. Compared to the window air conditioners these portable ones are more convenient.
A portable air conditioner is also a great money saving solution, as you only need to purchase one single unit, which can be easily placed wherever you need it. Some of the models weight as less as 36 pounds and are mounted on castors. Compared to the conventional air conditioning system, the portable models have low energy consumption as they cool only one room and not the entire house.
The portable air conditioners offer excellent energy efficiency. The efficiency ranges in average between 8 and 10, but it can also be as high as 15, meaning a 50 percent higher efficiency compared to older air conditioners. As portable air conditioners are small sized, they can be easily fitted against the wall or simply in a corner. Its size is comparable to a room dehumidifier and it actually functions like a dehumidifier.
The humidity of the room is reduced as a smaller model eliminates about 35 pints of moisture on a daily basis from the air of the room. They function based on the heat-exchange principle of the conventional air conditioning systems to cool the air. The air is cooled and dehumidified as the air is circulated through cold evaporator coils. The room air is eliminated through the hot condenser.
In our modern days, most portable air conditioner models include electronic controls allowing you to set the wanted room temperature, operation mode (heat, dehumidify or cool) and blower speed. Hand-held remote controls are also included with the portable air conditioners, enabling you to easily modify the settings with no effort whatsoever.
Portable air conditioners can also include various other features, such as an incorporated air cleaner. Optionally, you can select charcoal air cleaner filter and oscillating louvers, which enables you to distribute the cooled air within the room. The louvers can be switched off allowing the air to flow straight towards you, according to your individual needs.
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So I know that T-Fun world has been on some crazy hiatus lately but I want to come back and share some new great stories that I think will summarize how I am currently feeling about my impending new life coming into this world.
1. Reading the baby books.
I have am actually really proud of myself for taking the time to read the baby books and actually found one that has been both humorous and knowledgeable. I also would like to point out that my wife has actually not read any of the baby books. I can?t blame her cause ?What To Expect When You Are Expecting? really freaked her out. It made her so terrified of the pregnancy. I read ?Dad Is Pregnant Too? which is really funny and is a good read. I found myself going back to it when I found some of the material related to what was going on in the pregnancy. Unlike that Satan?s Guide to Pregnancy that my wife was too sacred to read, it showed me that pregnancy was not beautiful but really gross. I have found that the gas, mood swings, and the vomiting go with the territory.
2. Setting up the baby?s room
I actually started this a few weeks ago thinking of what I wanted to do. We got some of the furniture from our friends and family. We got a killer changing table from my sister in law?s employer. We even got a bassinet from one of my friends at work. I was so excited. Now I am just trying to figure out what to do with our computer desk cause let?s face it, it will be hard to blog if I have to worry about waking the baby up. I also have to pack up a bookshelf and move it out so I can make room to put the dresser my father gave us. The only thing left to do is just get a crib. My wife and I are almost there.
3. Finding alternate foods for dinner.
This has been really hard cause my wife and I have always based most of our meals around chicken but since she has been pregnant I am having to find other meats then the bird. This is one of the reasons I have not posted hardly any new recipes on the blog lately. Since my wife has been pregnant I have been having to make things like pork chops, hamburgers, and hotdogs. If those fail we usually go out to dinner. The cravings so far have been for late night trips for ice cream. When I say cravings most of them have not been from just her. It gets really frustrating when she tells you that salad taste like dirt, or could you grill it outside cause the smell is making me sick, or can you not make that cause it made me vomit twice the other day. I hope that one day I get to have fried chicken again. Someday.
4. Taking care of my wife.
Like I have said before I have become a master of the ?duck and cover? method when I see the nostrils flaring and the smoke coming from her ears. The hardest things is never knowing what will set her off. A few weeks ago my house became a World War 3 war zone when I questioned my wife on why she wanted to throw a gender reveal party. It was bad and I am still picking my teeth off the floor but I will manage.
Side Note: Gender Reveal Parties are actually really cool but more on that a little later.
It has not only been dodging bullets when she is mad either. It has also been when she is sad or upset. I have been a huge advocate for the destruction of The Lifetime Movie Channel since the beginning of this pregnancy cause my wife will just sit there and cry the entire time. I ask her why she is cry and she just tells me she has no idea why. Its been an emotional roller coaster from day one and we are not even half done with this pregnancy. Other times she tells me she is unhappy with the way she looks and she is just so tired of feeling sick. I then have to remind her that she is not herself and that she will ok and to think of the bigger picture.
5. Finding time for myself
This has not been this hard. I always have a day to myself every week to get things done around the house, catch up with my shows on the DVR, and have lunch with my dad. It gives me the tim to go to the gym and to man stuff which is always important. Sometimes i wish I could go out with the guys and get a few drinks. Tonight I was actually invited to a free Def Leppard concert. I had to turn it down cause my wife really wanted to spend some time with me. I feel that I need to get these fun times in now cause soon I am going to have a baby to worry about. How bad does it look if you bring your baby to a bar? Real bad? I know. That was a dumb question.
6. Advice from other dads.
I have found a lot of awesome advice from some dads I started following on Twitter. Not only are these dads helpful but they are also so funny. I find that when i get nervous they are always there to say pregnancy does get better but the real adventure will start soon enough. When I doubt my parental skills they are always there to tell me that no dad is perfect and you will make mistakes. I needed this and glad that I found some people to relate to. The other great thing about is that they don?t know you so they have no reason not to be honest.
7. Go to every doctor?s visit.
I don?t feel I have to say much about this cause I am very lucky to be able to go to every doctor?s visit . I am very thankful that it works into our schedule. I think that it is really important to be there for my wife. To hold her hand as we get to listen to the baby?s heartbeat and to ask the doctor question that my wife may have forgotten to ask. It just works better for everyone if you are there.
These are just a few of the things I have been doing to get ready for the birth of my child. We are currently in the middle of week 17 of our pregnancy. We are almost to the halfway mark. I do have a list of things I am not looking forward to but I will share them at a later date.
I'm the guy behind Buzzbin Magazine. Back in 2008 I put out my first issue of Buzzbin hoping to promote the local music scene in Akron/Canton. Since then we've grown to be the largest alternative press in the area. If you've met any of our crew then you know that we a bunch of hard workers with a passion to represent the community. Who says Akron/Canton/Cleveland is a miserable place to live? Not us. We have one of the best art scenes around, some of the hardest working musicians on the planet, and really great people to fill in everywhere else. We put out a new issue on THE FIRST WEDNESDAY OF EVERY MONTH covering the best art and entertainment found in northeast Ohio. We never miss a beat. Wonder why our mag is choked full of advertisers? Because we know what we're doing. We offer full scale marketing for each and every business that comes to us. We develop marketing campaigns from the ground up, or we'll take over where someone left off. We have the ability to tell everyone about you. And we're open for business. We're not pushy, we're helpful. - Mike (330) 236-5005
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Public release date: 13-Aug-2012
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